Theme 1
Microscopy & ImagingModern soft matter research is increasingly driven by high-resolution, high-throughput experimental techniques, and the first theme, Microscopy & Imaging, will introduce participants to state-of-the-art tools that allow direct visualization of structure, dynamics, and function across scales, from molecular assemblies to mesoscopic collective behavior.
The module will begin with elastic scattering techniques: Small-Angle X-ray Scattering (SAXS), Small-Angle Neutron Scattering (SANS), and Static Light Scattering (SLS), which provide reciprocal-space access to nanoscale organization and correlations, along with a brief introduction to inelastic scattering to highlight how dynamical information can be extracted. Real-space imaging will be discussed starting from the Abbe theory of image formation, establishing fundamental limits of optical resolution. Contrast-enhancing optical methods such as Phase Contrast, Differential Interference Contrast (DIC), and Dark Field microscopy will be introduced for visualizing transparent and weakly scattering soft materials. Fluorescence microscopy and super-resolution techniques will be covered to illustrate selective imaging and nanoscale visualization beyond the diffraction limit. The module will also include Atomic Force Microscopy (AFM) for nanoscale imaging and mechanical characterization, Optical Tweezers for force manipulation and micromechanical measurements, and Differential Dynamic Microscopy (DDM) as a powerful bridge between imaging and scattering for extracting dynamical information from time-resolved microscopy data.
Speakers:
- Prof. Roberto Cerbino – University of Vienna
- Prof. Pramod Pullarkat – Raman Research Institute
- Prof. V A Raghunathan – Raman Research Institute
- Prof. Shivprasad Patil – IISER Pune