Light Activated drug Delivery and Cancer Therapy

The emergence of light activated "optoporation" as a non-invasive technique for intracellular cargo delivery has revolutionized biomedical research and therapy. Optoporation uses precise laser-induced permeabilization of cell membranes, enabling efficient delivery of biomolecules such as siRNA, plasmids, and proteins. Current research focuses on enhancing delivery efficiency and safety by optimizing laser parameters and integrating nanomaterials to improve targeting therapy. Recent advancements also highlight the use of bioprinted tissue models and organoids to better mimic in vivo environments, providing a bridge between conventional in vitro studies and clinical applications. Despite significant progress, challenges such as;diffusion barriers in complex tissues and off-target effects in vivo remain key areas for innovation.

Aims and objectives:

1. Development of Optoporator;Tool: Design and optimize a laser activated Optoporator for scalable, minimally invasive, and efficient intracellular cargo delivery.

2. Validation on;Bioprinted Models: Evaluate the Optoporator’s efficacy in delivering therapeutic biomolecules to bioprinted tumor models replicating native tissue;architecture.

3. Preclinical in Vivo Testing: Assess safety, gene knockdown efficiency, and therapeutic efficacy in tumor-bearing mice to establish;translational potential.

4. Human Organoid Application: Investigate delivery efficiency, therapeutic outcomes, and paracrine effects in human cancerderived organoids, advancing personalized cancer therapy